Application of Spectrophotometric Methods for the Determination of Thiamine (VB1) in Pharmaceutical Formulations Using 7-Chloro-4-Nitrobenzoxadiazole (NBD-Cl)

Since Thiamine the first discovered vitamin among B complex vitamins is considered as an essential nutrient [1], and used for treatment of many disorders and diseases [2]; and because it is included as a component of different multi-vitamins, a variety of analytical methods have been proposed for its determination in pharmaceutical formulations. Those methods include flow injection analysis (FIA) [3-7], spectrophotometric [8] chromatographic methods [9,10], fluorometry [11], high-performance liquid chromatography (HPLC) [12,13], chemilumenescence [2], and electrochemical techniques [14,15].